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Landscape of X chromosome inactivation across human tissues

Author(s): Tukiainen, Taru; Villani, Alexandra-Chloé; Yen, Angela; Rivas, Manuel A; Marshall, Jamie L; et al

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dc.contributor.authorTukiainen, Taru-
dc.contributor.authorVillani, Alexandra-Chloé-
dc.contributor.authorYen, Angela-
dc.contributor.authorRivas, Manuel A-
dc.contributor.authorMarshall, Jamie L-
dc.contributor.authorSatija, Rahul-
dc.contributor.authorAguirre, Matt-
dc.contributor.authorGauthier, Laura-
dc.contributor.authorFleharty, Mark-
dc.contributor.authorKirby, Andrew-
dc.contributor.authorCummings, Beryl B-
dc.contributor.authorCastel, Stephane E-
dc.contributor.authorKarczewski, Konrad J-
dc.contributor.authorAguet, François-
dc.contributor.authorByrnes, Andrea-
dc.contributor.authorGTEx Consortium-
dc.contributor.authorLappalainen, Tuuli-
dc.contributor.authorRegev, Aviv-
dc.contributor.authorArdlie, Kristin G-
dc.contributor.authorHacohen, Nir-
dc.contributor.authorMacArthur, Daniel G-
dc.identifier.citationTukiainen, Taru, Alexandra-Chloé Villani, Angela Yen, Manuel A. Rivas, Jamie L. Marshall, Rahul Satija, Matt Aguirre. Laura Gauthier, Mark Fleharty, Andrew Kirby, Beryl B. Cummings, Stephane E. Castel, Konrad J. Karczewski, François Aguet, Andrea Byrnes, GTEx Consortium, Tuuli Lappalainen, Aviv Regev, Kristin G. Ardlie, Nir Hacohen, and Daniel G. MacArthur. "Landscape of X chromosome inactivation across human tissues." Nature 550, no. 7675 (2017): pp. 244-248. doi:10.1038/nature24265en_US
dc.description.abstractX chromosome inactivation (XCI) silences transcription from one of the two X chromosomes in female mammalian cells to balance expression dosage between XX females and XY males. XCI is, however, incomplete in humans: up to one-third of X-chromosomal genes are expressed from both the active and inactive X chromosomes (Xa and Xi, respectively) in female cells, with the degree of ‘escape’ from inactivation varying between genes and individuals1,2. The extent to which XCI is shared between cells and tissues remains poorly characterized3,4, as does the degree to which incomplete XCI manifests as detectable sex differences in gene expression5 and phenotypic traits6. Here we describe a systematic survey of XCI, integrating over 5,500 transcriptomes from 449 individuals spanning 29 tissues from GTEx (v6p release) and 940 single-cell transcriptomes, combined with genomic sequence data. We show that XCI at 683 X-chromosomal genes is generally uniform across human tissues, but identify examples of heterogeneity between tissues, individuals and cells. We show that incomplete XCI affects at least 23% of X-chromosomal genes, identify seven genes that escape XCI with support from multiple lines of evidence and demonstrate that escape from XCI results in sex biases in gene expression, establishing incomplete XCI as a mechanism that is likely to introduce phenotypic diversity6,7. Overall, this updated catalogue of XCI across human tissues helps to increase our understanding of the extent and impact of the incompleteness in the maintenance of XCI.en_US
dc.format.extent244 - 248en_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleLandscape of X chromosome inactivation across human tissuesen_US
dc.typeJournal Articleen_US

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