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RNA transcription modulates phase transition-driven nuclear body assembly

Author(s): Berry, J; Weber, SC; Vaidya, N; Haataja, M; Brangwynne, CP; et al

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dc.contributor.authorBerry, J-
dc.contributor.authorWeber, SC-
dc.contributor.authorVaidya, N-
dc.contributor.authorHaataja, M-
dc.contributor.authorBrangwynne, CP-
dc.contributor.authorWeitz, DA-
dc.identifier.citationBerry, J, Weber, SC, Vaidya, N, Haataja, M, Brangwynne, CP, Weitz, DA. (2015). RNA transcription modulates phase transition-driven nuclear body assembly. Proceedings of the National Academy of Sciences of the United States of America, 112 (E5237 - E5245. doi:10.1073/pnas.1509317112en_US
dc.description.abstractLiving cells contain various membraneless organelles whose size and assembly appear to be governed by equilibrium thermodynamic phase separation. However, the dynamics of this process are poorly understood. Here, we quantify the assembly dynamics of liquid-phase nuclear bodies and find that they can be explained by classical models of phase separation and coarsening. In addition, active nonequilibrium processes, particularly rRNA transcription, can locally modulate thermodynamic parameters to stabilize nucleoli. Our findings demonstrate that the classical phase separation mechanisms long associated with nonliving condensed matter can mediate organelle assembly in living cells, whereas chemical activity may serve to regulate these processes in response to developmental or environmental conditions.en_US
dc.format.extentE5237 - E5245en_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.rightsFinal published version. Article is made available in OAR by the publisher's permission or policy.en_US
dc.titleRNA transcription modulates phase transition-driven nuclear body assemblyen_US
dc.typeJournal Articleen_US

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