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|Abstract:||The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans' four major tissues (or "tissue-ome"), identifying ubiquitously expressed and tissue-specific "enriched" genes. These data newly reveal the hypodermis' metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms. Finally, we developed a machine learning-based prediction tool for 76 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-β activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals.|
|Citation:||Kaletsky, Rachel, Yao, Victoria, Williams, April, Runnels, Alexi M, Tadych, Alicja, Zhou, Shiyi, Troyanskaya, Olga G, Murphy, Coleen T. (2018). Transcriptome analysis of adult Caenorhabditis elegans cells reveals tissue-specific gene and isoform expression.. PLoS genetics, 14 (8), e1007559 - ?. doi:10.1371/journal.pgen.1007559|
|Pages:||e1007559 - ?|
|Type of Material:||Journal Article|
|Journal/Proceeding Title:||PLoS genetics|
|Version:||Final published version. This is an open access article.|
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