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Inhibition of stationary phase respiration impairs persister formation in E. coli

Author(s): Orman, Mehmet A; Brynildsen, Mark P

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dc.contributor.authorOrman, Mehmet A-
dc.contributor.authorBrynildsen, Mark P-
dc.date.accessioned2021-10-08T19:58:14Z-
dc.date.available2021-10-08T19:58:14Z-
dc.date.issued2015-08-06en_US
dc.identifier.citationOrman, Mehmet A, Brynildsen, Mark P. (2015). Inhibition of stationary phase respiration impairs persister formation in E. coli. Nature Communications, 6 (7983 - 7983. doi:10.1038/ncomms8983en_US
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr18z70-
dc.description.abstractBacterial persisters are rare phenotypic variants that temporarily tolerate high antibiotic concentrations. Persisters have been hypothesized to underlie the recalcitrance of biofilm infections, and strategies to eliminate these cells have the potential to improve treatment outcomes for many hospital-treated infections. Here we investigate the role of stationary phase metabolism in generation of type I persisters in Escherichia coli, which are those that are formed by passage through stationary phase. We find that persisters are unlikely to derive from bacteria with low redox activity, and that inhibition of respiration during stationary phase reduces persister levels by up to ∼1,000-fold. Loss of stationary phase respiratory activity prevents digestion of endogenous proteins and RNA, which yields bacteria that are more capable of translation, replication and concomitantly cell death when exposed to antibiotics. These findings establish bacterial respiration as a prime target for reducing the number of persisters formed in nutrient-depleted, non-growing populations.en_US
dc.language.isoen_USen_US
dc.relation.ispartofNature Communicationsen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleInhibition of stationary phase respiration impairs persister formation in E. colien_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1038/ncomms8983-
dc.date.eissued2015-08-06en_US
dc.identifier.eissn2041-1723-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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