# Domain prediction with probabilistic directional context.

## Author(s): Ochoa, Alejandro; Singh, Mona

To refer to this page use: http://arks.princeton.edu/ark:/88435/pr17v7h
 Abstract:

Motivation

Protein domain prediction is one of the most powerful approaches for sequence-based function prediction. Although domain instances are typically predicted independently of each other, newer approaches have demonstrated improved performance by rewarding domain pairs that frequently co-occur within sequences. However, most of these approaches have ignored the order in which domains preferentially co-occur and have also not modeled domain co-occurrence probabilistically.

Results

We introduce a probabilistic approach for domain prediction that models 'directional' domain context. Our method is the first to score all domain pairs within a sequence while taking their order into account, even for non-sequential domains. We show that our approach extends a previous Markov model-based approach to additionally score all pairwise terms, and that it can be interpreted within the context of Markov random fields. We formulate our underlying combinatorial optimization problem as an integer linear program, and demonstrate that it can be solved quickly in practice. Finally, we perform extensive evaluation of domain context methods and demonstrate that incorporating context increases the number of domain predictions by ∼15%, with our approach dPUC2 (Domain Prediction Using Context) outperforming all competing approaches.

Availability and implementation

dPUC2 is available at http://github.com/alexviiia/dpuc2.

Contact

mona@cs.princeton.edu.

Supplementary information

Supplementary data are available at Bioinformatics online. Publication Date: Aug-2017 Citation: Ochoa, Alejandro, Singh, Mona. (2017). Domain prediction with probabilistic directional context.. Bioinformatics (Oxford, England), 33 (16), 2471 - 2478. doi:10.1093/bioinformatics/btx221 DOI: doi:10.1093/bioinformatics/btx221 ISSN: 1367-4803 EISSN: 1367-4811 Pages: 2471 - 2478 Language: eng Type of Material: Journal Article Journal/Proceeding Title: Bioinformatics (Oxford, England) Version: Final published version. This is an open access article.