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Flow-scanning optical tomography

Author(s): Pégard, Nicolas C; Toth, Marton L; Driscoll, Monica; Fleischer, Jason W

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Abstract: We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes.
Publication Date: 7-Aug-2014
Citation: Pégard, Nicolas C, Toth, Marton L, Driscoll, Monica, Fleischer, Jason W. (Flow-scanning optical tomography. Lab Chip, 14 (23), 4447 - 4450. doi:10.1039/c4lc00701h
DOI: doi:10.1039/c4lc00701h
ISSN: 1473-0197
EISSN: 1473-0189
Pages: 4447 - 4450
Language: en
Type of Material: Journal Article
Journal/Proceeding Title: Lab on a Chip
Version: Author's manuscript

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