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Electron Microscopic Reconstruction of Functionally Identified Cells in a Neural Integrator

Author(s): Vishwanathan, Ashwin; Daie, Kayvon; Ramirez, Alexandro D; Lichtman, Jeff W; Aksay, Emre RF; et al

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dc.contributor.authorVishwanathan, Ashwin-
dc.contributor.authorDaie, Kayvon-
dc.contributor.authorRamirez, Alexandro D-
dc.contributor.authorLichtman, Jeff W-
dc.contributor.authorAksay, Emre RF-
dc.contributor.authorSeung, H Sebastian-
dc.date.accessioned2021-10-08T19:45:06Z-
dc.date.available2021-10-08T19:45:06Z-
dc.date.issued2017en_US
dc.identifier.citationVishwanathan, Ashwin, Kayvon Daie, Alexandro D. Ramirez, Jeff W. Lichtman, Emre RF Aksay, and H. Sebastian Seung. "Electron Microscopic Reconstruction of Functionally Identified Cells in a Neural Integrator." Current Biology 27, no. 14 (2017): 2137-2147.e3. doi:10.1016/j.cub.2017.06.028en_US
dc.identifier.issn0960-9822-
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569574/pdf/nihms891994.pdf-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr15g0r-
dc.descriptionSupplemental Information: https://www.cell.com/cms/10.1016/j.cub.2017.06.028/attachment/96c7f056-2ce2-42db-b620-208883903d09/mmc1.pdfen_US
dc.description.abstractNeural integrators are involved in a variety of sensorimotor and cognitive behaviors. The oculomotor system contains a simple example, a hindbrain neural circuit that takes velocity signals as inputs and temporally integrates them to control eye position. Here we investigated the structural underpinnings of temporal integration in the larval zebrafish by first identifying integrator neurons using two-photon calcium imaging and then reconstructing the same neurons through serial electron microscopic analysis. Integrator neurons were identified as those neurons with activities highly correlated with eye position during spontaneous eye movements. Three morphological classes of neurons were observed: ipsilaterally projecting neurons located medially, contralaterally projecting neurons located more laterally, and a population at the extreme lateral edge of the hindbrain for which we were not able to identify axons. Based on their somatic locations, we inferred that neurons with only ipsilaterally projecting axons are glutamatergic, whereas neurons with only contralaterally projecting axons are largely GABAergic. Dendritic and synaptic organization of the ipsilaterally projecting neurons suggests a broad sampling from inputs on the ipsilateral side. We also observed the first conclusive evidence of synapses between integrator neurons, which have long been hypothesized by recurrent network models of integration via positive feedback.en_US
dc.format.extent2137 - 2147.e3en_US
dc.language.isoen_USen_US
dc.relation.ispartofCurrent Biologyen_US
dc.rightsAuthor's manuscripten_US
dc.titleElectron Microscopic Reconstruction of Functionally Identified Cells in a Neural Integratoren_US
dc.typeJournal Articleen_US
dc.identifier.doi10.1016/j.cub.2017.06.028-
dc.identifier.eissn1879-0445-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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