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Volumetric two-photon imaging of neurons using stereoscopy (vTwINS)

Author(s): Song, Alexander; Charles, Adam S; Koay, Sue Ann; Gauthier, Jeff L; Thiberge, Stephan Y; et al

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Abstract: Two-photon laser scanning microscopy of calcium dynamics using fluorescent indicators is a widely used imaging method for large scale recording of neural activity in vivo. Here we introduce volumetric Two-photon Imaging of Neurons using Stereoscopy (vTwINS), a volumetric calcium imaging method that employs an elongated, V-shaped point spread function to image a 3D brain volume. Single neurons project to spatially displaced “image pairs” in the resulting 2D image, and the separation distance between images is proportional to depth in the volume. To demix the fluorescence time series of individual neurons, we introduce a novel orthogonal matching pursuit algorithm that also infers source locations within the 3D volume. We illustrate vTwINS by imaging neural population activity in mouse primary visual cortex and hippocampus. Our results demonstrate that vTwINS provides an effective method for volumetric two-photon calcium imaging that increases the number of neurons recorded while maintaining a high frame-rate.
Publication Date: Apr-2017
Electronic Publication Date: 20-Mar-2017
Citation: Song, Alexander, Charles, Adam S, Koay, Sue Ann, Gauthier, Jeff L, Thiberge, Stephan Y, Pillow, Jonathan W, Tank, David W. (2017). Volumetric two-photon imaging of neurons using stereoscopy (vTwINS). Nature Methods, 14 (4), 420 - 426. doi:10.1038/nmeth.4226
DOI: doi:10.1038/nmeth.4226
ISSN: 1548-7091
EISSN: 1548-7105
Pages: 420 - 426
Type of Material: Journal Article
Journal/Proceeding Title: Nature Methods
Version: Author's manuscript

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