Skip to main content

Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme

Author(s): Csefalvay, Eva; Lapkouski, Mikalai; Guzanova, Alena; Csefalvay, Ladislav; Baikova, Tatsiana; et al

Download
To refer to this page use: http://arks.princeton.edu/ark:/88435/pr1q518
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCsefalvay, Eva-
dc.contributor.authorLapkouski, Mikalai-
dc.contributor.authorGuzanova, Alena-
dc.contributor.authorCsefalvay, Ladislav-
dc.contributor.authorBaikova, Tatsiana-
dc.contributor.authorShevelev, Igor-
dc.contributor.authorBialevich, Vitali-
dc.contributor.authorShamayeva, Katsiaryna-
dc.contributor.authorJanscak, Pavel-
dc.contributor.authorKuta Smatanova, Ivana-
dc.contributor.authorPanjikar, Santosh-
dc.contributor.authorCarey, Jannette-
dc.contributor.authorWeiserova, Marie-
dc.contributor.authorEttrich, Rüdiger-
dc.date.accessioned2020-10-27T18:31:55Z-
dc.date.available2020-10-27T18:31:55Z-
dc.date.issued2015-06-03en_US
dc.identifier.citationCsefalvay, Eva, Lapkouski, Mikalai, Guzanova, Alena, Csefalvay, Ladislav, Baikova, Tatsiana, Shevelev, Igor, Bialevich, Vitali, Shamayeva, Katsiaryna, Janscak, Pavel, Kuta Smatanova, Ivana, Panjikar, Santosh, Carey, Jannette, Weiserova, Marie, Ettrich, Rüdiger. (2015). Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme. PLOS ONE, 10 (6), e0128700 - e0128700. doi:10.1371/journal.pone.0128700.en_US
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1q518-
dc.descriptionPLoS ONE. Volume 10, Issue 6, 3 June 2015, Article number e0128700.en_US
dc.description.abstractType I restriction-modification enzymes are multifunctional heteromeric complexes with DNA cleavage and ATP-dependent DNA translocation activities located on motor subunit HsdR. Functional coupling of DNA cleavage and translocation is a hallmark of the Type I restriction systems that is consistent with their proposed role in horizontal gene transfer. DNA cleavage occurs at nonspecific sites distant from the cognate recognition sequence, apparently triggered by stalled translocation. The X-ray crystal structure of the complete HsdR subunit from E. coli plasmid R124 suggested that the triggering mechanism involves interdomain contacts mediated by ATP. In the present work, in vivo and in vitro activity assays and crystal structures of three mutants of EcoR124I HsdR designed to probe this mechanism are reported. The results indicate that interdomain engagement via ATP is indeed responsible for signal transmission between the endonuclease and helicase domains of the motor subunit. A previously identified sequence motif that is shared by the RecB nucleases and some Type I endonucleases is implicated in signaling. © 2015 Csefalvay et al.en_US
dc.format.extent10.6:e0128700-1 - e0128700-21en_US
dc.language.isoenen_US
dc.relation.ispartofPLOS ONEen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleFunctional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzymeen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1371/journal.pone.0128700-
dc.date.eissued2015-06-03en_US
dc.identifier.eissn1932-6203-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

Files in This Item:
File Description SizeFormat 
journal.pone.0128700.PDF3.84 MBAdobe PDFView/Download
journal.pone.0128700.g001.PNG1.6 MBimage/pngThumbnail
View/Download


Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.