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Self-Assembly of Catenanes from Lasso Peptides

Author(s): Allen, Caitlin D.; Link, A. James

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Abstract: Lasso peptides exist naturally in a threaded state as [1]rotaxanes, and we reasoned that lasso peptides cleaved in their loop region could serve as building blocks for catenanes. Mutagenesis of the lasso peptide microcin J25 (MccJ25) with two cysteine residues followed by cleavage of the peptide with trypsin led to a [2]rotaxane structure that self-assembled into a [3]catenane and [4]catenanes at room temperature in aqueous solution. The [3]catenane represents the smallest ring size of a catenane comprised solely of polypeptide segments. The NMR structure of the [3]catenane was determined, suggesting that burial of hydrophobic residues may be a driving force for assembly of the catenane structure.
Publication Date: 2-Nov-2016
Electronic Publication Date: 21-Oct-2016
Citation: Allen, Caitlin D., Link, A. James. (2016). Self-Assembly of Catenanes from Lasso Peptides. Journal of the American Chemical Society, 138 (43), 14214 - 14217. doi:10.1021/jacs.6b09454
DOI: doi:10.1021/jacs.6b09454
ISSN: 0002-7863
EISSN: 1520-5126
Pages: 14214 - 14217
Type of Material: Journal Article
Journal/Proceeding Title: Journal of the American Chemical Society
Version: Author's manuscript

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