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De Novo Peptide Design with C3a Receptor Agonist and Antagonist Activities: Theoretical Predictions and Experimental Validation

Author(s): Bellows-Peterson, Meghan L; Fung, Ho Ki; Floudas, Christodoulos A; Kieslich, Chris A; Zhang, Li; et al

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dc.contributor.authorBellows-Peterson, Meghan L-
dc.contributor.authorFung, Ho Ki-
dc.contributor.authorFloudas, Christodoulos A-
dc.contributor.authorKieslich, Chris A-
dc.contributor.authorZhang, Li-
dc.contributor.authorMorikis, Dimitrios-
dc.contributor.authorWareham, Kathryn J-
dc.contributor.authorMonk, Peter N-
dc.contributor.authorHawksworth, Owen A-
dc.contributor.authorWoodruff, Trent M-
dc.identifier.citationBellows-Peterson, Meghan L., Ho Ki Fung, Christodoulos A. Floudas, Chris A. Kieslich, Li Zhang, Dimitrios Morikis, Kathryn J. Wareham, Peter N. Monk, Owen A. Hawksworth, and Trent M. Woodruff. "De novo peptide design with C3a receptor agonist and antagonist activities: theoretical predictions and experimental validation." Journal of Medicinal Chemistry 55, no. 9 (2012): 4159-4168. doi: 10.1021/jm201609ken_US
dc.description.abstractTargeting the complement component 3a receptor (C3aR) with selective agonists or antagonists is believed to be a viable therapeutic option for several diseases such as stroke, heart attack, reperfusion injuries, and rheumatoid arthritis. We designed a number of agonists, partial agonists, and antagonists of C3aR using our two-stage de novo protein design framework. Of the peptides tested using a degranulation assay in C3aR-transfected rat basophilic leukemia cells, two were prominent agonists (EC50 values of 25.3 and 66.2 nM) and two others were partial agonists (IC50 values of 15.4 and 26.1 nM). Further testing of these lead compounds in a calcium flux assay in U937 cells yielded similar results although with reduced potencies compared to transfected cells. The partial agonists also displayed full antagonist activity when tested in a C3aR inhibition assay. In addition, the electrostatic potential profile was shown to potentially discriminate between full agonists and partial agonists.en_US
dc.format.extent4159 - 4168en_US
dc.relation.ispartofJournal of Medicinal Chemistryen_US
dc.rightsAuthor's manuscripten_US
dc.titleDe Novo Peptide Design with C3a Receptor Agonist and Antagonist Activities: Theoretical Predictions and Experimental Validationen_US
dc.typeJournal Articleen_US

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