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Optogenetic control of protein binding using light-switchable nanobodies

Author(s): Gil, Agnieszka A; Carrasco-López, César; Zhu, Liyuan; Zhao, Evan M; Ravindran, Pavithran T; et al

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Abstract: A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few tools confer light-switchable binding to a generic target protein of interest. Such a capability would offer substantial advantages, enabling photoswitchable binding to endogenous target proteins in cells or light-based protein purification in vitro. Here, we report the development of opto-nanobodies (OptoNBs), a versatile class of chimeric photoswitchable proteins whose binding to proteins of interest can be enhanced or inhibited upon blue light illumination. We find that OptoNBs are suitable for a range of applications including reversibly binding to endogenous intracellular targets, modulating signaling pathway activity, and controlling binding to purified protein targets in vitro. This work represents a step towards programmable photoswitchable regulation of a wide variety of target proteins.
Publication Date: 13-Aug-2020
Citation: Gil, Agnieszka A, Carrasco-López, César, Zhu, Liyuan, Zhao, Evan M, Ravindran, Pavithran T, Wilson, Maxwell Z, Goglia, Alexander G, Avalos, José L, Toettcher, Jared E. (2020). Optogenetic control of protein binding using light-switchable nanobodies. Nature communications, 11 (1), 4044 - 4044. doi:10.1038/s41467-020-17836-8
DOI: doi:10.1038/s41467-020-17836-8
ISSN: 2041-1723
EISSN: 2041-1723
Pages: 4044 - 4044
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Nature Communications
Version: Final published version. This is an open access article.

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