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Promoting Neuronal Outgrowth Using Ridged Scaffolds Coated with Extracellular Matrix Proteins

Author(s): Siddiqui, Ahad M; Brunner, Rosa; Harris, Gregory M; Miller, Alan Lee; Waletzki, Brian E; et al

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Abstract: Spinal cord injury (SCI) results in cell death, demyelination, and axonal loss. The spinal cord has a limited ability to regenerate, and current clinical therapies for SCI are not effective in helping promote neurologic recovery. We have developed a novel scaffold biomaterial that is fabricated from the biodegradable hydrogel oligo(poly(ethylene glycol)fumarate) (OPF). We have previously shown that positively charged OPF scaffolds (OPF+) in an open spaced, multichannel design can be loaded with Schwann cells to support axonal generation and functional recovery following SCI. We have now developed a hybrid OPF+ biomaterial that increases the surface area available for cell attachment and that contains an aligned microarchitecture and extracellular matrix (ECM) proteins to better support axonal regeneration. OPF+ was fabricated as 0.08 mm thick sheets containing 100 μm high polymer ridges that self-assemble into a spiral shape when hydrated. Laminin, fibronectin, or collagen I coating promoted neuron attachment and axonal outgrowth on the scaffold surface. In addition, the ridges aligned axons in a longitudinal bipolar orientation. Decreasing the space between the ridges increased the number of cells and neurites aligned in the direction of the ridge. Schwann cells seeded on laminin coated OPF+ sheets aligned along the ridges over a 6-day period and could myelinate dorsal root ganglion neurons over 4 weeks. This novel scaffold design, with closer spaced ridges and Schwann cells, is a novel biomaterial construct to promote regeneration after SCI.
Publication Date: 27-Apr-2021
Citation: Siddiqui, Ahad M, Brunner, Rosa, Harris, Gregory M, Miller, Alan Lee, Waletzki, Brian E, Schmeichel, Ann M, Schwarzbauer, Jean E, Schwartz, Jeffrey, Yaszemski, Michael J, Windebank, Anthony J, Madigan, Nicolas N. (Promoting Neuronal Outgrowth Using Ridged Scaffolds Coated with Extracellular Matrix Proteins. Biomedicines, 9 (5), 479 - 479. doi:10.3390/biomedicines9050479
DOI: doi:10.3390/biomedicines9050479
EISSN: 2227-9059
Pages: 479 - 479
Language: en
Type of Material: Journal Article
Journal/Proceeding Title: Biomedicines
Version: Final published version. This is an open access article.

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