Skip to main content

Confinement size determines the architecture of Ran-induced microtubule networks

Author(s): Gai, Ya; Cook, Brian; Setru, Sagar; Stone, Howard A; Petry, Sabine

To refer to this page use:
Abstract: The organization of microtubules (MTs) within cells is critical for its internal organization during interphase and mitosis. During mitotic spindle assembly, MTs are made and organized around chromosomes in a process regulated by RanGTP. The role of RanGTP has been explored in Xenopus egg extracts, which are not limited by a cell membrane. Here, we investigated whether cell-sized confinements affect the assembly of RanGTP-induced MT networks in Xenopus egg extracts. We used microfluidics to encapsulate extract within monodisperse extract-in-oil droplets. Importantly, we find that the architecture of Ran-induced MT networks depends on the droplet diameter and the Ran concentration, and differs from structures formed in bulk extract. Our results highlight that both MT nucleation and physical confinement play critical roles in determining the spatial organization of the MT cytoskeleton.
Electronic Publication Date: 2021
Citation: Gai, Ya, Cook, Brian, Setru, Sagar, Stone, Howard A, Petry, Sabine. (Confinement size determines the architecture of Ran-induced microtubule networks. Soft Matter, 17 (24), 5921 - 5931. doi:10.1039/d1sm00045d
DOI: doi:10.1039/d1sm00045d
ISSN: 1744-683X
EISSN: 1744-6848
Language: en
Type of Material: Journal Article
Journal/Proceeding Title: Soft Matter
Version: Author's manuscript

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.