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Structural and Dynamical Features of Inteins and Implications on Protein Splicing

Author(s): Eryilmaz, Ertan; Shah, Neel H.; Muir, Thomas W.; Cowburn, David

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Abstract: Protein splicing is a posttranslational modification where intervening proteins (inteins) cleave themselves from larger precursor proteins and ligate their flanking polypeptides (exteins) through a multistep chemical reaction. First thought to be an anomaly found in only a few organisms, protein splicing by inteins has since been observed in microorganisms from all domains of life. Despite this broad phylogenetic distribution, all inteins share common structural features such as a horseshoelike pseudo two-fold symmetric fold, several canonical sequence motifs, and similar splicing mechanisms. Intriguingly, the splicing efficiencies and substrate specificity of different inteins vary considerably, reflecting subtle changes in the chemical mechanism of splicing, linked to their local structure and dynamics. As intein chemistry has widespread use in protein chemistry, understanding the structural and dynamical aspects of inteins is crucial for intein engineering and the improvement of inteinbased technologies.
Publication Date: 23-May-2014
Electronic Publication Date: 2-Apr-2014
Citation: Eryilmaz, Ertan, Shah, Neel H., Muir, Tom W., Cowburn, David. (2014). Structural and Dynamical Features of Inteins and Implications on Protein Splicing. Journal of Biological Chemistry, 289 (21), 14506 - 14511. doi:10.1074/jbc.R113.540302
DOI: doi:10.1074/jbc.R113.540302
ISSN: 0021-9258
EISSN: 1083-351X
Pages: 289.21:024504,14506-145111
Type of Material: Journal Article
Journal/Proceeding Title: Journal of Biological Chemistry
Version: Final published version. This is an open access article.
Notes: Eryilma, E., Shah, N. H., Muir, T. W., & Cowburn, D. (2014). Structural and dynamical features of Inteins and implications on protein splicing. Journal of Biological Chemistry, 289(21), 14506-14511. Access the most updated version of this article at doi: 10.1074/jbc.R113.540302. Authors: Ertan Eryilmaz, Neel H. Shah, Muir, Thomas W. (Muir,Tom), and David Cowburn.

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