Skip to main content

Fluid dynamics in zebrafish Kupffer's vesicle

Author(s): Okabe, Noriko; Xu, Bo; Burdine, Rebecca

To refer to this page use:
Abstract: Work in mouse has implicated cilia motility and leftward nodal flow as the mechanism for breaking symmetry. In zebrafish, it is assumed that Kupffer’s vesicle is analogous to the mouse node. However, its architecture is different and the fluid dynamics inside Kupffer’s vesicle is not completely understood. We show that cells lining both the dorsal roof and the ventral floor of Kupffer’s vesicle possess posteriorly pointed cilia that rotate clockwise. Analysis of bead movements within Kupffer’s vesicle shows a net circular flow but the local flow differs in direction depending on the location within the vesicle. Histological analysis suggests that the orientation of the cells at anterior-dorsal region likely direct net flow in the vesicle. Our data suggest that the plane of the circular net flow is tilted with respect to the D-V axis, which may be converted to a local leftward flow in the anteriordorsal region of the vesicle.
Publication Date: 2008
Electronic Publication Date: 20-Nov-2008
Citation: Okabe, Noriko, Burdine, Rebecca D. (2008). Fluid dynamics in zebrafish Kupffer's vesicle. Developmental Biology, 319 (2), 579 - 579. doi:10.1016/j.ydbio.2008.05.398
DOI: doi:10.1016/j.ydbio.2008.05.398
ISSN: 0012-1606
Pages: 3602-3612
Type of Material: Journal Article
Journal/Proceeding Title: Developmental Biology
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.