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The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.

Author(s): Freeman Rosenzweig, Elizabeth S; Xu, Bin; Kuhn Cuellar, Luis; Martinez-Sanchez, Antonio; Schaffer, Miroslava; et al

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Abstract: Approximately 30%-40% of global CO2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number" effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.
Publication Date: 21-Sep-2017
Citation: Freeman Rosenzweig, Elizabeth S, Xu, Bin, Kuhn Cuellar, Luis, Martinez-Sanchez, Antonio, Schaffer, Miroslava, Strauss, Mike, Cartwright, Heather N, Ronceray, Pierre, Plitzko, Jürgen M, Förster, Friedrich, Wingreen, Ned S, Engel, Benjamin D, Mackinder, Luke CM, Jonikas, Martin C. (2017). The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.. Cell, 171 (1), 148 - 162.e19. doi:10.1016/j.cell.2017.08.008
DOI: doi:10.1016/j.cell.2017.08.008
ISSN: 0092-8674
EISSN: 1097-4172
Pages: 1 - 40
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Cell
Version: Author's manuscript

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