Skip to main content

The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.

Author(s): Freeman Rosenzweig, Elizabeth S; Xu, Bin; Kuhn Cuellar, Luis; Martinez-Sanchez, Antonio; Schaffer, Miroslava; et al

Download
To refer to this page use: http://arks.princeton.edu/ark:/88435/pr1f20z
Full metadata record
DC FieldValueLanguage
dc.contributor.authorFreeman Rosenzweig, Elizabeth S-
dc.contributor.authorXu, Bin-
dc.contributor.authorKuhn Cuellar, Luis-
dc.contributor.authorMartinez-Sanchez, Antonio-
dc.contributor.authorSchaffer, Miroslava-
dc.contributor.authorStrauss, Mike-
dc.contributor.authorCartwright, Heather N-
dc.contributor.authorRonceray, Pierre-
dc.contributor.authorPlitzko, Jürgen M-
dc.contributor.authorFörster, Friedrich-
dc.contributor.authorWingreen, Ned S-
dc.contributor.authorEngel, Benjamin D-
dc.contributor.authorMackinder, Luke CM-
dc.contributor.authorJonikas, Martin C-
dc.date.accessioned2020-02-25T20:11:35Z-
dc.date.available2020-02-25T20:11:35Z-
dc.date.issued2017-09-21en_US
dc.identifier.citationFreeman Rosenzweig, Elizabeth S, Xu, Bin, Kuhn Cuellar, Luis, Martinez-Sanchez, Antonio, Schaffer, Miroslava, Strauss, Mike, Cartwright, Heather N, Ronceray, Pierre, Plitzko, Jürgen M, Förster, Friedrich, Wingreen, Ned S, Engel, Benjamin D, Mackinder, Luke CM, Jonikas, Martin C. (2017). The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.. Cell, 171 (1), 148 - 162.e19. doi:10.1016/j.cell.2017.08.008en_US
dc.identifier.issn0092-8674-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1f20z-
dc.description.abstractApproximately 30%-40% of global CO2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number" effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.en_US
dc.format.extent1 - 40en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofCellen_US
dc.rightsAuthor's manuscripten_US
dc.titleThe Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization.en_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1016/j.cell.2017.08.008-
dc.identifier.eissn1097-4172-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

Files in This Item:
File Description SizeFormat 
The Eukaryotic CO2-Concentrating Organelle is Liquid-Like and Exhibits Dynamic Reorganization.pdf4.82 MBAdobe PDFView/Download


Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.