Skip to main content

Hierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicum

Author(s): Aristilde, Ludmilla

Download
To refer to this page use: http://arks.princeton.edu/ark:/88435/pr1dz5j
Full metadata record
DC FieldValueLanguage
dc.contributor.authorAristilde, Ludmilla-
dc.date.accessioned2020-11-12T18:51:42Z-
dc.date.available2020-11-12T18:51:42Z-
dc.date.issued2015-02-15en_US
dc.identifier.citationAristilde, Ludmilla, Lewis, Ian A., Park, Junyoung O., Rabinowitz, Joshua D. (2015). Hierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicum. Applied and Environmental Microbiology, 81 (4), 1452 - 1462. doi:10.1128/AEM.03199-14en_US
dc.identifier.issn0099-2240-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1dz5j-
dc.description.abstractBacterial metabolism of polysaccharides from plant detritus into acids and solvents is an essential component of the terrestrial carbon cycle. Understanding the underlying metabolic pathways can also contribute to improved production of biofuels. Using a metabolomics approach involving liquid chromatography-mass spectrometry, we investigated the metabolism of mixtures of the cellulosic hexose sugar (glucose) and hemicellulosic pentose sugars (xylose and arabinose) in the anaerobic soil bacterium Clostridium acetobutylicum. Simultaneous feeding of stable isotope-labeled glucose and unlabeled xylose or arabinose revealed that, as expected, glucose was preferentially used as the carbon source. Assimilated pentose sugars accumulated in pentose phosphate pathway (PPP) intermediates with minimal flux into glycolysis. Simultaneous feeding of xylose and arabinose revealed an unexpected hierarchy among the pentose sugars, with arabinose utilized preferentially over xylose. The phosphoketolase pathway (PKP) provides an alternative route of pentose catabolism in C. acetobutylicum that directly converts xylulose-5-phosphate into acetyl-phosphate and glyceraldehyde-3-phosphate, bypassing most of the PPP. When feeding the mixture of pentose sugars, the labeling patterns of lower glycolytic intermediates indicated more flux through the PKP than through the PPP and upper glycolysis, and this was confirmed by quantitative flux modeling. Consistent with direct acetyl-phosphate production from the PKP, growth on the pentose mixture resulted in enhanced acetate excretion. Taken collectively, these findings reveal two hierarchies in clostridial pentose metabolism: xylose is subordinate to arabinose, and the PPP is used less than the PKP.en_US
dc.format.extent1452 - 1462en_US
dc.language.isoen_USen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.rightsFinal published version. Article is made available in OAR by the publisher's permission or policy.en_US
dc.titleHierarchy in Pentose Sugar Metabolism in Clostridium acetobutylicumen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1128/AEM.03199-14-
dc.date.eissued2014-12-19en_US
dc.identifier.eissn1098-5336-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

Files in This Item:
File Description SizeFormat 
1452.full.pdf3.2 MBAdobe PDFView/Download


Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.