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A Review on Quantitative Multiplexed Proteomics

Author(s): Pappireddi, Nishant; Martin, Lance; Wühr, Martin

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Abstract: Over the last few decades, mass spectrometry-based proteomics has become an increasingly powerful tool that is now able to routinely detect and quantify thousands of proteins. A major advance for global protein quantification was the introduction of isobaric tags, which, in a single experiment, enabled the global quantification of proteins across multiple samples. Herein, these methods are referred to as multiplexed proteomics. The principles, advantages, and drawbacks of various multiplexed proteomics techniques are discussed and compared with alternative approaches. We also discuss how the emerging combination of multiplexing with targeted proteomics might enable the reliable and high-quality quantification of very low abundance proteins across multiple conditions. Lastly, we suggest that fusing multiplexed proteomics with data-independent acquisition approaches might enable the comparison of hundreds of different samples without missing values, while maintaining the superb measurement precision and accuracy obtainable with isobaric tag quantification.
Publication Date: May-2019
Citation: Pappireddi, Nishant, Martin, Lance, Wühr, Martin. (2019). A Review on Quantitative Multiplexed Proteomics. Chembiochem : a European journal of chemical biology, 20 (10), 1210 - 1224. doi:10.1002/cbic.201800650
DOI: doi:10.1002/cbic.201800650
ISSN: 1439-4227
EISSN: 1439-7633
Pages: 1210 - 1224
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Chembiochem : a European journal of Chemical Biology
Version: Author's manuscript

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