Skip to main content

A versatile optical microscope for time-dependent single-molecule and single-particle spectroscopy

Author(s): Li, Hao; Yang, Haw

To refer to this page use:
Abstract: © 2018 Author(s). This work reports the design and implementation of a multi-function optical microscope for time-dependent spectroscopy on single molecules and single nanoparticles. It integrates the now-routine single-object measurements into one standalone platform so that no reconfiguration is needed when switching between different types of sample or spectroscopy modes. The illumination modes include evanescent field through total internal reflection, dark-field illumination, and epi-excitation onto a diffraction-limited spot suitable for confocal detection. The detection modes include spectrally resolved line imaging, wide-field imaging with dual-color capability, and two-color single-element photon-counting detection. The switch between different spectroscopy and data acquisition modes is fully automated and executed through computer programming. The capability of this microscope is demonstrated through selected proof-of-principle experiments.
Publication Date: 28-Mar-2018
Electronic Publication Date: 2-Jan-2018
Citation: Li, H., Yang, H. (2018). A versatile optical microscope for time-dependent single-molecule and single-particle spectroscopy. Journal of Chemical Physics, 148 (12), 10.1063/1.5009134
DOI: doi:10.1063/1.5009134
ISSN: 0021-9606
Pages: 148, 123316-1 - 123316-11
Type of Material: Journal Article
Journal/Proceeding Title: Journal of Chemical Physics
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.