Skip to main content

A size-invariant bud-duration timer enables robustness in yeast cell size control

Author(s): Allard, Corey AH; Decker, Franziska; Weiner, Orion; Toettcher, Jared; Graziano, Brian

To refer to this page use:
Abstract: Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant ‘timer’ specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.
Publication Date: 21-Dec-2018
Citation: Allard, Corey AH, Franziska Decker, Orion D. Weiner, Jared E. Toettcher, and Brian R. Graziano. "A size-invariant bud-duration timer enables robustness in yeast cell size control." PloS one 13, no. 12 (2018): e0209301.
DOI: doi:10.1371/journal.pone.0209301
ISSN: 1932-6203
Pages: e0209301
Type of Material: Journal Article
Journal/Proceeding Title: PLoS ONE
Version: Final published version. This is an open access article.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.