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A size-invariant bud-duration timer enables robustness in yeast cell size control

Author(s): Allard, Corey AH; Decker, Franziska; Weiner, Orion; Toettcher, Jared; Graziano, Brian

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dc.contributor.authorAllard, Corey AH-
dc.contributor.authorDecker, Franziska-
dc.contributor.authorWeiner, Orion-
dc.contributor.authorToettcher, Jared-
dc.contributor.authorGraziano, Brian-
dc.date.accessioned2022-01-25T14:48:17Z-
dc.date.available2022-01-25T14:48:17Z-
dc.date.issued2018-12-21en_US
dc.identifier.citationAllard, Corey AH, Franziska Decker, Orion D. Weiner, Jared E. Toettcher, and Brian R. Graziano. "A size-invariant bud-duration timer enables robustness in yeast cell size control." PloS one 13, no. 12 (2018): e0209301.en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr18c49-
dc.description.abstractCell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant ‘timer’ specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.en_US
dc.format.extente0209301en_US
dc.language.isoen_USen_US
dc.relation.ispartofPLoS ONEen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleA size-invariant bud-duration timer enables robustness in yeast cell size controlen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1371/journal.pone.0209301-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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