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Long-term hepatitis B infection in a scalable hepatic co-culture system.

Author(s): Winer, Benjamin Y.; Huang, Tiffany S.; Pludwinski, Eitan; Heller, Brigitte; Wojcik, Felix; et al

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Abstract: Hepatitis B virus causes chronic infections in 250 million people worldwide. Chronic hepatitis B virus carriers are at risk of developing fibrosis, cirrhosis, and hepatocellular carcinoma. A prophylactic vaccine exists and currently available antivirals can suppress but rarely cure chronic infections. The study of hepatitis B virus and development of curative antivirals are hampered by a scarcity of models that mimic infection in a physiologically relevant, cellular context. Here, we show that cell-culture and patient-derived hepatitis B virus can establish persistent infection for over 30 days in a self-assembling, primary hepatocyte co-culture system. Importantly, infection can be established without antiviral immune suppression, and susceptibility is not donor dependent. The platform is scalable to microwell formats, and we provide proof-of-concept for its use in testing entry inhibitors and antiviral compounds.The lack of models that mimic hepatitis B virus (HBV) infection in a physiologically relevant context has hampered drug development. Here, Winer et al. establish a self-assembling, primary hepatocyte co-culture system that can be infected with patient-derived HBV without further modifications.
Publication Date: 25-Jul-2017
Citation: Winer, Benjamin Y., Huang, Tiffany S., Pludwinski, Eitan, Heller, Brigitte, Wojcik, Felix, Lipkowitz, Gabriel E., Parekh, Amit, Cho, Cheul, Shrirao, Anil, Muir, Tom W., Novik, Eric, Ploss, Alexander. (2017). Long-term hepatitis B infection in a scalable hepatic co-culture system.. Nature communications, 8 (1), 125 - ?. doi:10.1038/s41467-017-00200-8
DOI: doi:10.1038/s41467-017-00200-8
ISSN: 2041-1723
EISSN: 2041-1723
Pages: 8.1:125,1-11
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Nature communications
Version: Final published version. This is an open access article.
Notes: Volume 8, Issue 1, 1 December 2017, Article number 125, pgs. 1-11. Authors: Benjamin Y. Winer, Tiffany S. Huang, Eitan Pludwinski, Brigitte Heller, Felix Wojcik, Gabriel E. Lipkowitz, Amit Parekh, Cheul Cho, Anil Shrirao, Thomas W. Muir (Tom W. Muir), Eric Novik & Alexander Ploss.

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