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RpoS proteolysis is controlled directly by ATP levels in Escherichia coli

Author(s): Peterson, Celeste N; Levchenko, Igor; Rabinowitz, Joshua D; Baker, Tania A; Silhavy, Thomas J

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Abstract: The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degradation in vivo. Nutrient upshifts trigger RpoS degradation independently of protein synthesis by activating metabolic pathways that generate small energy molecules. Using metabolic mutants and inhibitors, we show that ATP, but not GTP or NADH, is necessary for RpoS degradation. In vitro reconstitution assays directly demonstrate that ClpXP fails to degrade RpoS, but not other proteins, at low ATP hydrolysis rates. These data suggest that cellular ATP levels directly control RpoS stability.
Publication Date: 15-Mar-2012
Electronic Publication Date: 16-Mar-2012
Citation: Peterson, CN, Levchenko, I, Rabinowitz, JD, Baker, TA, Silhavy, TJ. (2012). RpoS proteolysis is controlled directly by ATP levels in Escherichia coli. Genes & Development, 26 (6), 548 - 553. doi:10.1101/gad.183517.111
DOI: doi:10.1101/gad.183517.111
ISSN: 0890-9369
Pages: 548 - 553
Type of Material: Journal Article
Journal/Proceeding Title: Genes & Development
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

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