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Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in Pseudomonas aeruginosa.

Author(s): Høyland-Kroghsbo, Nina Molin; Muñoz, Katrina Arcelia; Bassler, Bonnie L

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dc.contributor.authorHøyland-Kroghsbo, Nina Molin-
dc.contributor.authorMuñoz, Katrina Arcelia-
dc.contributor.authorBassler, Bonnie L-
dc.date.accessioned2020-02-25T20:11:29Z-
dc.date.available2020-02-25T20:11:29Z-
dc.date.issued2018-11-13en_US
dc.identifier.citationHøyland-Kroghsbo, Nina Molin, Muñoz, Katrina Arcelia, Bassler, Bonnie L. (2018). Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in Pseudomonas aeruginosa.. mBio, 9 (6), 10.1128/mBio.02184-18en_US
dc.identifier.issn2150-7511-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1zz1d-
dc.description.abstractClustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) systems are adaptive defense systems that protect bacteria and archaea from invading genetic elements. In Pseudomonas aeruginosa, quorum sensing (QS) induces the CRISPR-Cas defense system at high cell density when the risk of bacteriophage infection is high. Here, we show that another cue, temperature, modulates P. aeruginosa CRISPR-Cas. Increased CRISPR adaptation occurs at environmental (i.e., low) temperatures compared to that at body (i.e., high) temperature. This increase is a consequence of the accumulation of CRISPR-Cas complexes, coupled with reduced P. aeruginosa growth rate at the lower temperature, the latter of which provides additional time prior to cell division for CRISPR-Cas to patrol the cell and successfully eliminate and/or acquire immunity to foreign DNA. Analyses of a QS mutant and synthetic QS compounds show that the QS and temperature cues act synergistically. The diversity and level of phage encountered by P. aeruginosa in the environment exceed that in the human body, presumably warranting increased reliance on CRISPR-Cas at environmental temperatures.en_US
dc.format.extent1 - 12en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofmBioen_US
dc.rightsFinal published version. Article is made available in OAR by the publisher's permission or policy.en_US
dc.titleTemperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in Pseudomonas aeruginosa.en_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1128/mBio.02184-18-
dc.identifier.eissn2150-7511-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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