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Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion

Author(s): Smith, Jean A; Hall, Allison E; Rose, Mark D

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Abstract: Cell fusion is ubiquitous in eukaryotic fertilization and development. The highly conserved Rho-GTPase Cdc42p promotes yeast fusion through interaction with Fus2p, a pheromone-induced amphiphysin-like protein. We show that in prezygotes, Cdc42p forms a novel Fus2p-dependent focus at the center of the zone of cell fusion (ZCF) and remains associated with remnant cell walls after initial fusion. At the ZCF and during fusion, Cdc42p and Fus2p colocalized. In contrast, in shmoos, both proteins were near the cortex but spatially separate. Cdc42p focus formation depends on ZCF membrane curvature: mutant analysis showed that Cdc42p localization is negatively affected by shmoo-like positive ZCF curvature, consistent with the flattening of the ZCF during fusion. BAR-domain proteins such as the fusion proteins Fus2p and Rvs161p are known to recognize membrane curvature. We find that mutations that disrupt binding of the Fus2p/Rvs161p heterodimer to membranes affect Cdc42p ZCF localization. We propose that Fus2p localizes Cdc42p to the flat ZCF to promote cell wall degradation.
Publication Date: Dec-2017
Citation: Smith, Jean A, Hall, Allison E, Rose, Mark D. (2017). Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion. The Journal of Cell Biology, 216 (12), 3971 - 3980. doi:10.1083/jcb.201703169
DOI: doi:10.1083/jcb.201703169
ISSN: 0021-9525
EISSN: 1540-8140
Pages: 3971 - 3980
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: The Journal of Cell Biology
Version: Final published version. This is an open access article.

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