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Quantitative 4D analyses of epithelial folding during Drosophila gastrulation

Author(s): Khan, Zia; Wang, Yu-Chiun; Wieschaus, Eric F; Kaschube, Matthias

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Abstract: Understanding the cellular and mechanical processes that underlie the shape changes of individual cells and their collective behaviors in a tissue during dynamic and complex morphogenetic events is currently one of the major frontiers in developmental biology. The advent of highspeed time-lapse microscopy and its use in monitoring the cellular events in fluorescently labeled developing organisms demonstrate tremendous promise in establishing detailed descriptions of these events and could potentially provide a foundation for subsequent hypothesis-driven research strategies. However, obtaining quantitative measurements of dynamic shapes and behaviors of cells and tissues in a rapidly developing metazoan embryo using time-lapse 3D microscopy remains technically challenging, with the main hurdle being the shortage of robust imaging processing and analysis tools.We have developed EDGE4D, a software tool for segmenting and tracking membrane-labeled cells using multi-photon microscopy data. Our results demonstrate that EDGE4D enables quantification of the dynamics of cell shape changes, cell interfaces and neighbor relations at single-cell resolution during a complex epithelial folding event in the early Drosophila embryo. We expect this tool to be broadly useful for the analysis of epithelial cell geometries and movements in a wide variety of developmental contexts.
Publication Date: 15-Jul-2014
Electronic Publication Date: 19-Jun-2014
Citation: Khan, Zia, Wang, Yu-Chiun, Wieschaus, Eric F, Kaschube, Matthias. (2014). Quantitative 4D analyses of epithelial folding during Drosophila gastrulation. Development, 141 (14), 2895 - 2900. doi:10.1242/dev.107730
DOI: doi:10.1242/dev.107730
ISSN: 0950-1991
EISSN: 1477-9129
Pages: 2895 - 2900
Type of Material: Journal Article
Journal/Proceeding Title: Development
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

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