Skip to main content

c21orf59/kurly Controls Both Cilia Motility and Polarization

Author(s): Jaffe, Kimberly M.; Grimes, Daniel T.; Schottenfeld-Roames, Jodi; Werner, Michael E.; Ku, Tse-Shuen J.; et al

Download
To refer to this page use: http://arks.princeton.edu/ark:/88435/pr1z19g
Abstract: Cilia are microtubule-based projections that function in the movement of extracellular fluid. This requires cilia to be: (1) motile and driven by dynein complexes and (2) correctly polarized on the surface of cells, which requires planar cell polarity (PCP). Few factors that regulate both processes have been discovered. We reveal that C21orf59/Kurly (Kur), a cytoplasmic protein with some enrichment at the base of cilia, is needed for motility; zebrafish mutants exhibit characteristic developmental abnormalities and dynein arm defects. kur was also required for proper cilia polarization in the zebrafish kidney and the larval skin of Xenopus laevis. CRISPR/Cas9 coupled with homologous recombination to disrupt the endogenous kur locus in Xenopus resulted in the asymmetric localization of the PCP protein Prickle2 being lost in mutant multiciliated cells. Kur also makes interactions with other PCP components, including Disheveled. This supports a model wherein Kur plays a dual role in cilia motility and polarization.
Publication Date: Mar-2016
Citation: Jaffe, Kimberly M, Grimes, Daniel T, Schottenfeld-Roames, Jodi, Werner, Michael E, Ku, Tse-Shuen J, Kim, Sun K, Pelliccia, Jose L, Morante, Nicholas FC, Mitchell, Brian J, Burdine, Rebecca D. (2016). c21orf59/kurly Controls Both Cilia Motility and Polarization. Cell Reports, 14 (8), 1841 - 1849. doi:10.1016/j.celrep.2016.01.069
DOI: doi:10.1016/j.celrep.2016.01.069
ISSN: 2211-1247
Pages: 1841 - 1849
Type of Material: Journal Article
Journal/Proceeding Title: Cell Reports
Version: Final published version. This is an open access article.



Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.