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Posttranslational Control of Cdc25 Degradation Terminates Drosophila’s Early Cell-Cycle Program

Author(s): Di Talia, Stefano; She, Richard; Blythe, Shelby A; Lu, Xuemin; Zhang, Qi Fan; et al

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Abstract: In most metazoans, early embryonic development is characterized by rapid mitotic divisions that are controlled by maternal mRNAs and proteins that accumulate during oogenesis [1]. These rapid divisions pause at the Mid-Blastula Transition (MBT), coinciding with a dramatic increase in gene transcription and the degradation of a subset of maternal mRNAs [2, 3]. In Drosophila, the cell cycle pause is controlled by inhibitory phosphorylation of Cdk1, which in turn is driven by downregulation of the activating Cdc25 phosphatases [4, 5]. Here, we show that the two Drosophila Cdc25 homologues, String and Twine, differ in their dynamics and that, contrary to current models [4], their down-regulations are not controlled by mRNA degradation but through different post-translational mechanisms. The degradation rate of String protein gradually increases during the late syncytial cycles in a manner dependent on the nuclear-to-cytoplasmic ratio and on the DNA replication checkpoints. Twine, on the other hand, is targeted for degradation at the onset of the MBT through a switch-like mechanism controlled like String by the nuclear-to-cytoplasmic ratio, but not requiring the DNA replication checkpoints. We demonstrate that post-translational control of Twine degradation ensures that the proper number of mitoses precede the MBT
Publication Date: 21-Jan-2013
Electronic Publication Date: 3-Jan-2013
Citation: Di Talia, Stefano, She, Richard, Blythe, Shelby A, Lu, Xuemin, Zhang, Qi Fan, Wieschaus, Eric F. (2013). Posttranslational Control of Cdc25 Degradation Terminates Drosophila’s Early Cell-Cycle Program. Current Biology, 23 (2), 127 - 132. doi:10.1016/j.cub.2012.11.029
DOI: doi:10.1016/j.cub.2012.11.029
ISSN: 0960-9822
Pages: 127 - 132
Type of Material: Journal Article
Journal/Proceeding Title: Current Biology
Version: Author's manuscript

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