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An approach for long-term, multi-probe Neuropixels recordings in unrestrained rats

Author(s): Luo, Thomas Zhihao; Bondy, Adrian Gopnik; Gupta, Diksha; Elliott, Verity Alexander; Kopec, Charles D; et al

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Abstract: The use of Neuropixels probes for chronic neural recordings is in its infancy and initial studies leave questions about long-term stability and probe reusability unaddressed. Here, we demonstrate a new approach for chronic Neuropixels recordings over a period of months in freely moving rats. Our approach allows multiple probes per rat and multiple cycles of probe reuse. We found that hundreds of units could be recorded for multiple months, but that yields depended systematically on anatomical position. Explanted probes displayed a small increase in noise compared to unimplanted probes, but this was insufficient to impair future single-unit recordings. We conclude that cost-effective, multi-region, and multi-probe Neuropixels recordings can be carried out with high yields over multiple months in rats or other similarly sized animals. Our methods and observations may facilitate the standardization of chronic recording from Neuropixels probes in freely moving animals.
Publication Date: 22-Oct-2020
Citation: Luo, Thomas Zhihao, Bondy, Adrian Gopnik, Gupta, Diksha, Elliott, Verity Alexander, Kopec, Charles D, Brody, Carlos D. (2020). An approach for long-term, multi-probe Neuropixels recordings in unrestrained rats. eLife, 9 (10.7554/elife.59716)
DOI: doi:10.7554/elife.59716
ISSN: 2050-084X
EISSN: 2050-084X
Pages: e59716 - e59716
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

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