Skip to main content

Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles

Author(s): Ding, Qiang; Heller, Brigitte; Capuccino, Juan MV; Song, Bokai; Nimgaonkar, Ila; et al

Download
To refer to this page use: http://arks.princeton.edu/ark:/88435/pr1vh5ch6c
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDing, Qiang-
dc.contributor.authorHeller, Brigitte-
dc.contributor.authorCapuccino, Juan MV-
dc.contributor.authorSong, Bokai-
dc.contributor.authorNimgaonkar, Ila-
dc.contributor.authorHrebikova, Gabriela-
dc.contributor.authorContreras, Jorge E-
dc.contributor.authorPloss, Alexander-
dc.date.accessioned2022-01-25T14:57:53Z-
dc.date.available2022-01-25T14:57:53Z-
dc.date.issued2017-01-31en_US
dc.identifier.citationDing, Qiang, Heller, Brigitte, Capuccino, Juan MV, Song, Bokai, Nimgaonkar, Ila, Hrebikova, Gabriela, Contreras, Jorge E, Ploss, Alexander. (2017). Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles. Proceedings of the National Academy of Sciences, 114 (5), 1147 - 1152. doi:10.1073/pnas.1614955114en_US
dc.identifier.issn0027-8424-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1vh5ch6c-
dc.description.abstractHepatitis E virus (HEV) is the leading cause of enterically transmitted viral hepatitis globally. Of HEV’s three ORFs, the function of ORF3 has remained elusive. Here, we demonstrate that via homophilic interactions ORF3 forms multimeric complexes associated with intracellular endoplasmic reticulum (ER)-derived membranes. HEV ORF3 shares several structural features with class I viroporins, and the function of HEV ORF3 can be maintained by replacing it with the well-characterized viroporin influenza A virus (IAV) matrix-2 protein. ORF3’s ion channel function is further evidenced by its ability to mediate ionic currents when expressed in Xenopus laevis oocytes. Furthermore, we identified several positions in ORF3 critical for its formation of multimeric complexes, ion channel activity, and, ultimately, release of infectious particles. Collectively, our data demonstrate a previously undescribed function of HEV ORF3 as a viroporin, which may serve as an attractive target in developing direct-acting antiviralsen_US
dc.format.extent1147 - 1152en_US
dc.language.isoen_USen_US
dc.relation.ispartofProceedings of the National Academy of Sciencesen_US
dc.rightsFinal published version. Article is made available in OAR by the publisher's permission or policy.en_US
dc.titleHepatitis E virus ORF3 is a functional ion channel required for release of infectious particlesen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1073/pnas.1614955114-
dc.date.eissued2017-01-17en_US
dc.identifier.eissn1091-6490-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

Files in This Item:
File Description SizeFormat 
Hepatitis_E_ORF3_infectious_particles.pdf1.5 MBAdobe PDFView/Download


Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.