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A lipoprotein/beta-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane

Author(s): Konovalova, Anna; Mitchell, Angela M; Silhavy, Thomas J

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Abstract: Lipoprotein RcsF is the OM component of the Rcs envelope stress response. RcsF exists in complexes with b-barrel proteins (OMPs) allowing it to adopt a transmembrane orientation with a lipidated N-terminal domain on the cell surface and a periplasmic C-terminal domain. Here we report that mutations that remove BamE or alter a residue in the RcsF trans-lumen domain specifically prevent assembly of the interlocked complexes without inactivating either RcsF or the OMP. Using these mutations we demonstrate that these RcsF/OMP complexes are required for sensing OM outer leaflet stress. Using mutations that alter the positively charged surface-exposed domain, we show that RcsF monitors lateral interactions between lipopolysaccharide (LPS) molecules. When these interactions are disrupted by cationic antimicrobial peptides, or by the loss of negatively charged phosphate groups on the LPS molecule, this information is transduced to the RcsF C-terminal signaling domain located in the periplasm to activate the stress response.
Publication Date: 10-Jun-2016
Citation: Konovalova, Anna, Mitchell, Angela M, Silhavy, Thomas J. (2016). A lipoprotein/beta-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane. eLife, 5 (10.7554/eLife.15276)
DOI: doi:10.7554/eLife.15276
ISSN: 2050-084X
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.



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