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Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA

Author(s): Zhou, Ruobo; Zhang, Jichuan; Bochman, Matthew L; Zakian, Virginia A; Ha, Taekjip

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Abstract: Pif1 family helicases are conserved from bacteria to humans. Here, we report a novel DNA patrolling activity which may underlie Pif1’s diverse functions: a Pif1 monomer preferentially anchors itself to a 3′-tailed DNA junction and periodically reel in the 3′ tail with a step size of one nucleotide, extruding a loop. This periodic patrolling activity is used to unfold an intramolecular G-quadruplex (G4) structure on every encounter, and is sufficient to unwind RNA-DNA heteroduplex but not duplex DNA. Instead of leaving after G4 unwinding, allowing it to refold, or going beyond to unwind duplex DNA, Pif1 repeatedly unwinds G4 DNA, keeping it unfolded. Pif1-induced unfolding of G4 occurs in three discrete steps, one strand at a time, and is powerful enough to overcome G4-stabilizing drugs. The periodic patrolling activity may keep Pif1 at its site of in vivo action in displacing telomerase, resolving R-loops, and keeping G4 unfolded during replication, recombination and repair.
Publication Date: 29-Apr-2014
Electronic Publication Date: 29-Apr-2014
Citation: Zhou, Ruobo, Zhang, Jichuan, Bochman, Matthew L, Zakian, Virginia A, Ha, Taekjip. (2014). Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA. eLife, 3 (10.7554/eLife.02190)
DOI: doi:10.7554/eLife.02190
EISSN: 2050-084X
Pages: e02190 - e02190
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

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