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Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos.

Author(s): Momen-Roknabadi, Amir; Di Talia, Stefano; Wieschaus, Eric

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Abstract: The development of an embryo requires precise spatiotemporal regulation of cellular processes. During Drosophila gastrulation, a precise temporal pattern of cell division is encoded through transcriptional regulation of cdc25(string) in 25 distinct mitotic domains. Using a genetic screen, we demonstrate that the same transcription factors that regulate the spatial pattern of cdc25(string) transcription encode its temporal activation. We identify buttonhead and empty spiracles as the major activators of cdc25(string) expression in mitotic domain 2. The effect of these activators is balanced through repression by hairy, sloppy paired 1, and huckebein. Within the mitotic domain, temporal precision of mitosis is robust and unaffected by changing dosage of rate-limiting transcriptional factors. However, precision can be disrupted by altering the levels of the two activators or two repressors. We propose that the additive and balanced action of activators and repressors is a general strategy for precise temporal regulation of cellular transitions during development.
Publication Date: 13-Sep-2016
Electronic Publication Date: 13-Sep-2016
Citation: Momen-Roknabadi, Amir, Di Talia, Stefano, Wieschaus, Eric. (2016). Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos.. Cell reports, 16 (11), 2793 - 2801. doi:10.1016/j.celrep.2016.08.034
DOI: doi:10.1016/j.celrep.2016.08.034
ISSN: 2211-1247
EISSN: 2211-1247
Pages: 2793 - 2801
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Cell Reports
Version: Final published version. This is an open access article.

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