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Guidelines for morpholino use in zebrafish

Author(s): Stainier, Didier Y. R.; Raz, Erez; Lawson, Nathan D.; Ekker, Stephen C.; Burdine, Rebecca D.; et al

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Abstract: The zebrafish (Danio rerio) has emerged as a powerful model to study vertebrate development and disease. Its short generation time makes it amenable to genetic manipulation and analysis, and its small size and high fecundity make it especially well suited for large-scale forward genetic and chemical screens. Fast-developing zebrafish embryos are transparent, facilitating live imaging of a variety of developmental processes in wild-type and mutant animals. The zebrafish was originally chosen as a model with forward genetics in mind and has been used successfully in countless genetic screens. However, it quickly became a priority to be able to disrupt the function of specific genes in a targeted way. In the early 2000s, in the absence of tools for efficient targeted mutagenesis, morpholino (MO) antisense oligomers became the tool of choice for gene knockdown in a range of models including Xenopus, zebrafish, sea urchin, and chick [1, 2]. MOs are chemically synthesized oligomers that are typically injected into embryos at the 1-cell stage, bind complementary target mRNAs, and prevent their translation or splicing. They are similar to small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) in that they interfere with the function of a gene without altering its sequence.
Publication Date: 19-Oct-2017
Citation: Stainier, Didier YR, Raz, Erez, Lawson, Nathan D, Ekker, Stephen C, Burdine, Rebecca D, Eisen, Judith S, Ingham, Philip W, Schulte-Merker, Stefan, Yelon, Deborah, Weinstein, Brant M, Mullins, Mary C, Wilson, Stephen W, Ramakrishnan, Lalita, Amacher, Sharon L, Neuhauss, Stephan CF, Meng, Anming, Mochizuki, Naoki, Panula, Pertti, Moens, Cecilia B. (2017). Guidelines for morpholino use in zebrafish.. PLoS genetics, 13 (10), e1007000 (1 - 5). doi:10.1371/journal.pgen.1007000
DOI: doi:10.1371/journal.pgen.1007000
ISSN: 1553-7390
EISSN: 1553-7404
Pages: 1 - 5
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: PLoS Genetics
Version: Final published version. This is an open access article.

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