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Germ Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation

Author(s): Lerit, Dorothy A; Shebelut, Conrad W; Lawlor, Kristen J; Rusan, Nasser M; Gavis, Elizabeth R; et al

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Abstract: The primordial germ cells (PGCs) specified during embryogenesis serve as progenitors to the adult germline stem cells. In Drosophila, the proper specification and formation of PGCs require both centrosomes and germ plasm, which contains the germline determinants. Centrosomes are microtubule (MT)-organizing centers that ensure the faithful segregation of germ plasm into PGCs. To date, mechanisms that modulate centrosome behavior to engineer PGC development have remained elusive. Only one germ plasm component, Germ cell-less (Gcl), is known to play a role in PGC formation. Here, we show that Gcl engineers PGC formation by regulating centrosome dynamics. Loss of gcl leads to aberrant centrosome separation and elaboration of the astral MT network, resulting in inefficient germ plasm segregation and aborted PGC cellularization. Importantly, compromising centrosome separation alone is sufficient to mimic the gcl loss-of-function phenotypes. We conclude Gcl functions as a key regulator of centrosome separation required for proper PGC development.
Publication Date: 24-Jan-2017
Citation: Lerit, Dorothy A, Shebelut, Conrad W, Lawlor, Kristen J, Rusan, Nasser M, Gavis, Elizabeth R, Schedl, Paul, Deshpande, Girish. (2017). Germ Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation. Cell Reports, 18 (4), 831 - 839. doi:10.1016/j.celrep.2016.12.074
DOI: doi:10.1016/j.celrep.2016.12.074
ISSN: 2211-1247
Pages: 831 - 839
Type of Material: Journal Article
Journal/Proceeding Title: Cell Reports
Version: Final published version. This is an open access article.

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