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Visualization of Transvection in Living Drosophila Embryos.

Author(s): Lim, Bomyi; Heist, Tyler; Levine, Michael; Fukaya, Takashi

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Abstract: How remote enhancers interact with appropriate target genes persists as a central mystery in gene regulation. Here, we exploit the properties of transvection to explore enhancer-promoter communication between homologous chromosomes in living Drosophila embryos. We successfully visualized the activation of an MS2-tagged reporter gene by a defined developmental enhancer located in trans on the other homolog. This trans-homolog activation depends on insulator DNAs, which increase the stability-but not the frequency-of homolog pairing. A pair of heterotypic insulators failed to mediate transvection, raising the possibility that insulator specificity underlies the formation of chromosomal loop domains. Moreover, we found that a shared enhancer co-activates separate PP7 and MS2 reporter genes incis and intrans. Transvecting alleles weakly compete with one another, raising the possibility that they share a common pool of the transcription machinery. We propose that transvecting alleles form a trans-homolog "hub," which serves as a scaffold for the accumulation of transcription complexes.
Publication Date: Apr-2018
Citation: Lim, Bomyi, Heist, Tyler, Levine, Michael, Fukaya, Takashi. (2018). Visualization of Transvection in Living Drosophila Embryos.. Molecular Cell, 70 (2), 287 - 296.e6. doi:10.1016/j.molcel.2018.02.029
DOI: doi:10.1016/j.molcel.2018.02.029
ISSN: 1097-2765
EISSN: 1097-4164
Pages: 287 - 296.e6
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Molecular Cell
Version: Author's manuscript

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