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Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: IV. viral proteinase slides along DNA to locate and process its substrates.

Author(s): Blainey, Paul C.; Graziano, Vito; Pérez-Berná, Ana J.; McGrath, William J.; Flint, S. Jane; et al

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dc.contributor.authorBlainey, Paul C.-
dc.contributor.authorGraziano, Vito-
dc.contributor.authorPérez-Berná, Ana J.-
dc.contributor.authorMcGrath, William J.-
dc.contributor.authorFlint, S. Jane-
dc.contributor.authorSan Martín, Carmen-
dc.contributor.authorXie, X. Sunney-
dc.contributor.authorMangel, Walter F.-
dc.date.accessioned2020-02-27T22:31:34Z-
dc.date.available2020-02-27T22:31:34Z-
dc.date.issued2012-10-07en_US
dc.identifier.citationBlainey, Paul C, Graziano, Vito, Pérez-Berná, Ana J, McGrath, William J, Flint, S Jane, San Martín, Carmen, Xie, X Sunney, Mangel, Walter F. (2013). Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: IV. viral proteinase slides along DNA to locate and process its substrates.. The Journal of biological chemistry, 288 (3), 2092 - 2102. doi:10.1074/jbc.M112.407460en_US
dc.identifier.issn0021-9258-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1n787-
dc.description.abstractPrecursor proteins used in the assembly of adenovirus virions must be processed by the virally encoded adenovirus proteinase (AVP) before the virus particle becomes infectious. An activated adenovirus proteinase, the AVP-pVIc complex, was shown to slide along viral DNA with an extremely fast one-dimensional diffusion constant, 21.0 ± 1.9 × 10(6) bp(2)/s. In principle, one-dimensional diffusion can provide a means for DNA-bound proteinases to locate and process DNA-bound substrates. Here, we show that this is correct. In vitro, AVP-pVIc complexes processed a purified virion precursor protein in a DNA-dependent reaction; in a quasi in vivo environment, heat-disrupted ts-1 virions, AVP-pVIc complexes processed five different precursor proteins in DNA-dependent reactions. Sliding of AVP-pVIc complexes along DNA illustrates a new biochemical mechanism by which a proteinase can locate its substrates, represents a new paradigm for virion maturation, and reveals a new way of exploiting the surface of DNA.en_US
dc.format.extent1 - 14en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.relation.ispartofThe Journal of Biological Chemistryen_US
dc.rightsFinal published version. Article is made available in OAR by the publisher's permission or policy.en_US
dc.titleRegulation of a viral proteinase by a peptide and DNA in one-dimensional space: IV. viral proteinase slides along DNA to locate and process its substrates.en_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1074/jbc.M112.407460-
dc.identifier.eissn1083-351X-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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