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Phospho-Rasputin Stabilization by Sec16 Is Required for Stress Granule Formation upon Amino Acid Starvation

Author(s): Aguilera-Gomez, Angelica; Zacharogianni, Margarita; van Oorschot, Marinke M; Genau, Heide; Grond, Rianne; et al

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dc.contributor.authorAguilera-Gomez, Angelica-
dc.contributor.authorZacharogianni, Margarita-
dc.contributor.authorvan Oorschot, Marinke M-
dc.contributor.authorGenau, Heide-
dc.contributor.authorGrond, Rianne-
dc.contributor.authorVeenendaal, Tineke-
dc.contributor.authorSinsimer, Kristina S-
dc.contributor.authorGavis, Elizabeth R-
dc.contributor.authorBehrends, Christian-
dc.contributor.authorRabouille, Catherine-
dc.identifier.citationAguilera-Gomez, Angelica, Zacharogianni, Margarita, van Oorschot, Marinke M, Genau, Heide, Grond, Rianne, Veenendaal, Tineke, Sinsimer, Kristina S, Gavis, Elizabeth R, Behrends, Christian, Rabouille, Catherine. (2017). Phospho-Rasputin Stabilization by Sec16 Is Required for Stress Granule Formation upon Amino Acid Starvation. Cell reports, 20 (4), 935 - 948. doi:10.1016/j.celrep.2017.06.042en_US
dc.description.abstractMost cellular stresses induce protein translation inhibition and stress granule formation. Here, using Drosophila S2 cells, we investigate the role of G3BP/Rasputin in this process. In contrast to arsenite treatment, where dephosphorylated Ser142 Rasputin is recruited to stress granules, we find that, upon amino acid starvation, only the phosphorylated Ser142 form is recruited. Furthermore, we identify Sec16, a component of the endoplasmic reticulum exit site, as a Rasputin interactor and stabilizer. Sec16 depletion results in Rasputin degradation and inhibition of stress granule formation. However, in the absence of Sec16, pharmacological stabilization of Rasputin is not enough to rescue the assembly of stress granules. This is because Sec16 specifically interacts with phosphorylated Ser142 Rasputin, the form required for stress granule formation upon amino acid starvation. Taken together, these results demonstrate that stress granule formation is fine-tuned by specific signaling cues that are unique to each stress. These results also expand the role of Sec16 as a stress response protein.en_US
dc.format.extent935 - 948en_US
dc.relation.ispartofCell Reportsen_US
dc.rightsAuthor's manuscripten_US
dc.titlePhospho-Rasputin Stabilization by Sec16 Is Required for Stress Granule Formation upon Amino Acid Starvationen_US
dc.typeJournal Articleen_US

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