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Separating Functions of the Phage-Encoded Quorum-Sensing-Activated Antirepressor Qtip

Author(s): Silpe, Justin E; Bridges, Andrew A; Huang, Xiuliang; Coronado, Daniela R; Duddy, Olivia P; et al

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Abstract: Quorum sensing is a process of chemical communication that bacteria use to track cell density and coordinate gene expression across a population. Bacteria-infecting viruses, called phages, can encode quorum-sensing components that enable them to integrate host cell density information into the lysis-lysogeny decision. Vibriophage VP882 is one such phage, and activation of its quorum-sensing pathway leads to the production of an antirepressor called Qtip. Qtip interferes with the prophage repressor (cIVP882), leading to host-cell lysis. Here, we show that Qtip interacts with the N terminus of cIVP882, inhibiting both cIVP882 DNA binding and cIVP882 autoproteolysis. Qtip also sequesters cIVP882, localizing it to the poles. Qtip can localize to the poles independently of cIVP882. Alanine-scanning mutagenesis of Qtip shows that its localization and interference with cIVP882 activities are separable. Comparison of Qtip to a canonical phage antirepressor reveals that despite both proteins interacting with their partner repressors, only Qtip drives polar localization.
Publication Date: 8-Apr-2020
Electronic Publication Date: 25-Feb-2020
Citation: Silpe, Justin E, Bridges, Andrew A, Huang, Xiuliang, Coronado, Daniela R, Duddy, Olivia P, Bassler, Bonnie L. (2020). Separating Functions of the Phage-Encoded Quorum-Sensing-Activated Antirepressor Qtip.. Cell Host & Microbe, 27 (4), 629 - 641.e4. doi:10.1016/j.chom.2020.01.024
DOI: doi:10.1016/j.chom.2020.01.024
ISSN: 1931-3128
EISSN: 1934-6069
Pages: 629 - 641.e4
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Cell Host & Microbe
Version: Author's manuscript

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