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Single cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface Firmicutes population

Author(s): Labonté, Jessica M; Field, Erin K; Lau, Maggie; Chivian, Dylan; Van Heerden, Esta; et al

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dc.contributor.authorLabonté, Jessica M-
dc.contributor.authorField, Erin K-
dc.contributor.authorLau, Maggie-
dc.contributor.authorChivian, Dylan-
dc.contributor.authorVan Heerden, Esta-
dc.contributor.authorWommack, K Eric-
dc.contributor.authorKieft, Thomas L-
dc.contributor.authorOnstott, Tullis C-
dc.contributor.authorStepanauskas, Ramunas-
dc.date.accessioned2022-01-25T14:49:54Z-
dc.date.available2022-01-25T14:49:54Z-
dc.date.issued2015-04-22en_US
dc.identifier.citationLabonte, Jessica M., Erin K. Field, Maggie Lau, Dylan Chivian, Esta Van Heerden, K. Eric Wommack, Thomas L. Kieft, Tullis C. Onstott, and Ramunas Stepanauskas. "Single cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface Firmicutes population." Frontiers in Microbiology 6 (2015): 349. doi:10.3389/fmicb.2015.00349.en_US
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1mg7fv22-
dc.description.abstractA major fraction of Earth's prokaryotic biomass dwells in the deep subsurface, where cellular abundances per volume of sample are lower, metabolism is slower, and generation times are longer than those in surface terrestrial and marine environments. How these conditions impact biotic interactions and evolutionary processes is largely unknown. Here we employed single cell genomics to analyze cell-to-cell genome content variability and signatures of horizontal gene transfer (HGT) and viral infections in five cells of Candidatus Desulforudis audaxviator, which were collected from a 3 km-deep fracture water in the 2.9 Ga-old Witwatersrand Basin of South Africa. Between 0 and 32% of genes recovered from single cells were not present in the original, metagenomic assembly of Desulforudis, which was obtained from a neighboring subsurface fracture. We found a transposable prophage, a retron, multiple clustered regularly interspaced short palindromic repeats (CRISPRs) and restriction-modification systems, and an unusually high frequency of transposases in the analyzed single cell genomes. This indicates that recombination, HGT and viral infections are prevalent evolutionary events in the studied population of microorganisms inhabiting a highly stable deep subsurface environment.en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.relation.ispartofFrontiers in Microbiologyen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleSingle cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface Firmicutes populationen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.3389/fmicb.2015.00349-
dc.identifier.eissn1664-302X-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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