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Establishment and maintenance of heritable chromatin structure during early Drosophila embryogenesis

Author(s): Blythe, Shelby A; Wieschaus, Eric F

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Abstract: During embryogenesis, the initial chromatin state is established during a period of rapid proliferative activity. We have measured with 3-min time resolution how heritable patterns of chromatin structure are initially established and maintained during the midblastula transition (MBT). We find that regions of accessibility are established sequentially, where enhancers are opened in advance of promoters and insulators. These open states are stably maintained in highly condensed mitotic chromatin to ensure faithful inheritance of prior accessibility status across cell divisions. The temporal progression of establishment is controlled by the biological timers that control the onset of the MBT. In general, acquisition of promoter accessibility is controlled by the biological timer that measures the nucleo-cytoplasmic (N:C) ratio, whereas timing of enhancer accessibility is regulated independently of the N:C ratio. These different timing classes each associate with binding sites for two transcription factors, GAGA-factor and Zelda, previously implicated in controlling chromatin accessibility at ZGA.
Publication Date: 23-Nov-2016
Electronic Publication Date: 23-Nov-2016
Citation: Blythe, Shelby A, Wieschaus, Eric F. (2016). Establishment and maintenance of heritable chromatin structure during early Drosophila embryogenesis. eLife, 5 (10.7554/eLife.20148
DOI: doi:10.7554/eLife.20148
EISSN: 2050-084X
Pages: e20148 - e20148
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

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