A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.

Sigoillot, Frederic D; Lyman, Susan; Huckins, Jeremy F; Adamson, Britt; Chung, Eunah; Quattrochi, Brian; King, Randall W

A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.

Author(s): Sigoillot, Frederic D; Lyman, Susan; Huckins, Jeremy F; Adamson, Britt; Chung, Eunah; et al

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DC Field	Value	Language
dc.contributor.author	Sigoillot, Frederic D	-
dc.contributor.author	Lyman, Susan	-
dc.contributor.author	Huckins, Jeremy F	-
dc.contributor.author	Adamson, Britt	-
dc.contributor.author	Chung, Eunah	-
dc.contributor.author	Quattrochi, Brian	-
dc.contributor.author	King, Randall W	-
dc.date.accessioned	2020-02-25T20:11:06Z	-
dc.date.available	2020-02-25T20:11:06Z	-
dc.date.issued	2012-02-19	en_US
dc.identifier.citation	Sigoillot, Frederic D, Lyman, Susan, Huckins, Jeremy F, Adamson, Britt, Chung, Eunah, Quattrochi, Brian, King, Randall W. (2012). A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.. Nature methods, 9 (4), 363 - 366. doi:10.1038/nmeth.1898	en_US
dc.identifier.issn	1548-7091	-
dc.identifier.uri	http://arks.princeton.edu/ark:/88435/pr1k188	-
dc.description.abstract	Because off-target effects hamper interpretation and validation of RNAi screen data, we developed a bioinformatics method, genome-wide enrichment of seed sequence matches (GESS), to identify candidate off-targeted transcripts in primary screening data. GESS analysis revealed a prominent off-targeted transcript in several screens, including MAD2 (MAD2L1) in a screen for genes required for the spindle assembly checkpoint. GESS analysis results can enhance the validation rate in RNAi screens.	en_US
dc.format.extent	1 - 7	en_US
dc.language	eng	en_US
dc.language.iso	en	en_US
dc.relation.ispartof	Nature methods	en_US
dc.rights	Final published version. Article is made available in OAR by the publisher's permission or policy.	en_US
dc.title	A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.	en_US
dc.type	Journal Article	en_US
dc.identifier.doi	doi:10.1038/nmeth.1898	-
dc.identifier.eissn	1548-7105	-
pu.type.symplectic	http://www.symplectic.co.uk/publications/atom-terms/1.0/journal-article	en_US

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