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Mechanism of bidirectional thermotaxis in Escherichia coli

Author(s): Paulick, Anja; Jakovljevic, Vladimir; Zhang, SiMing; Erickstad, Michael; Groisman, Alex; et al

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Abstract: In bacteria various tactic responses are mediated by the same cellular pathway, but sensing of physical stimuli remains poorly understood. Here, we combine an in-vivo analysis of the pathway activity with a microfluidic taxis assay and mathematical modeling to investigate the thermotactic response of Escherichia coli. We show that in the absence of chemical attractants E. coli exhibits a steady thermophilic response, the magnitude of which decreases at higher temperatures. Adaptation of wildtype cells to high levels of chemoattractants sensed by only one of the major chemoreceptors leads to inversion of the thermotactic response at intermediate temperatures and bidirectional cell accumulation in a thermal gradient. A mathematical model can explain this behavior based on the saturation-dependent kinetics of adaptive receptor methylation. Lastly, we find that the preferred accumulation temperature corresponds to optimal growth in the presence of the chemoattractant serine, pointing to a physiological relevance of the observed thermotactic behavior.
Publication Date: 3-Aug-2017
Citation: Paulick, Anja, Jakovljevic, Vladimir, Zhang, SiMing, Erickstad, Michael, Groisman, Alex, Meir, Yigal, Ryu, William, Wingreen, Ned S, Sourjik, Victor. (2017). Mechanism of bidirectional thermotaxis in Escherichia coli. ELIFE, 6 (10.7554/eLife.26607)
DOI: doi:10.7554/eLife.26607
ISSN: 2050-084X
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

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