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RodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis

Author(s): Morgenstein, Randy M.; Bratton, Benjamin P.; Nguyen, Jeffrey P.; Ouzounov, Nikolay; Shaevitz, Joshua W.; et al

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Abstract: The rod shape of most bacteria requires the actin homolog, MreB. Whereas MreB was initially thought to statically define rod shape, recent studies found that MreB dynamically rotates around the cell circumference dependent on cell wall synthesis. However, the mechanism by which cytoplasmic MreB is linked to extracytoplasmic cell wall synthesis and the function of this linkage for morphogenesis has remained unclear. Here we demonstrate that the transmembrane protein RodZ mediates MreB rotation by directly or indirectly coupling MreB to cell wall synthesis enzymes. Furthermore, we map the RodZ domains that link MreB to cell wall synthesis and identify mreB mutants that suppress the shape defect of ΔrodZ without restoring rotation, uncoupling rotation from rod-like growth. Surprisingly, MreB rotation is dispensable for rodlike shape determination under standard laboratory conditions but is required for the robustness of rod shape and growth under conditions of cell wall stress.
Publication Date: 6-Oct-2015
Electronic Publication Date: 22-Sep-2015
Citation: Morgenstein, Randy M., Bratton, Benjamin P., Nguyen, Jeffrey P., Ouzounov, Nikolay, Shaevitz, Joshua W., Gitai, Zemer. (2015). RodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis. Proceedings of the National Academy of Sciences, 112 (40), 12510 - 12515. doi:10.1073/pnas.1509610112
DOI: doi:10.1073/pnas.1509610112
ISSN: 0027-8424
EISSN: 1091-6490
Pages: 12510 - 12515
Type of Material: Journal Article
Journal/Proceeding Title: Proceedings of the National Academy of Sciences
Version: Final published version. This is an open access article.

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