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Concentration dependent chromatin states induced by the bicoid morphogen gradient.

Author(s): Hannon, Colleen E; Blythe, Shelby A; Wieschaus, Eric F

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Abstract: In Drosophila, graded expression of the maternal transcription factor Bicoid (Bcd) provides positional information to activate target genes at different positions along the anterior-posterior axis. We have measured the genome-wide binding profile of Bcd using ChIP-seq in embryos expressing single, uniform levels of Bcd protein, and grouped Bcd-bound targets into four classes based on occupancy at different concentrations. By measuring the biochemical affinity of target enhancers in these classes in vitro and genome-wide chromatin accessibility by ATAC-seq, we found that the occupancy of target sequences by Bcd is not primarily determined by Bcd binding sites, but by chromatin context. Bcd drives an open chromatin state at a subset of its targets. Our data support a model where Bcd influences chromatin structure to gain access to concentration-sensitive targets at high concentrations, while concentration-insensitive targets are found in more accessible chromatin and are bound at low concentrations. This may be a common property of developmental transcription factors that must gain early access to their target enhancers while the chromatin state of the genome is being remodeled during large-scale transitions in the gene regulatory landscape.
Publication Date: 11-Sep-2017
Electronic Publication Date: 2-Aug-2017
Citation: Hannon, Colleen E, Blythe, Shelby A, Wieschaus, Eric F. (2017). Concentration dependent chromatin states induced by the bicoid morphogen gradient.. eLife, 6 (10.7554/elife.28275
DOI: doi:10.7554/elife.28275
ISSN: 2050-084X
EISSN: 2050-084X
Pages: e28275 - e28275
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

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