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Assembly of the algal CO2-fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif

Author(s): Meyer, Moritz T; Itakura, Alan K; Patena, Weronika; Wang, Lianyong; He, Shan; et al

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Abstract: Approximately one-third of the Earth's photosynthetic CO<sub>2</sub> assimilation occurs in a pyrenoid, an organelle containing the CO<sub>2</sub>-fixing enzyme Rubisco. How constituent proteins are recruited to the pyrenoid and how the organelle's subcompartments-membrane tubules, a surrounding phase-separated Rubisco matrix, and a peripheral starch sheath-are held together is unknown. Using the model alga <i>Chlamydomonas reinhardtii</i>, we found that pyrenoid proteins share a sequence motif. We show that the motif is necessary and sufficient to target proteins to the pyrenoid and that the motif binds to Rubisco, suggesting a mechanism for targeting. The presence of the Rubisco-binding motif on proteins that localize to the tubules and on proteins that localize to the matrix-starch sheath interface suggests that the motif holds the pyrenoid's three subcompartments together. Our findings advance our understanding of pyrenoid biogenesis and illustrate how a single protein motif can underlie the architecture of a complex multilayered phase-separated organelle.
Publication Date: 11-Nov-2020
Citation: Meyer, Moritz T, Itakura, Alan K, Patena, Weronika, Wang, Lianyong, He, Shan, Emrich-Mills, Tom, Lau, Chun S, Yates, Gary, Mackinder, Luke CM, Jonikas, Martin C. (2020). Assembly of the algal CO<sub>2</sub>-fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif. Science advances, 6 (46), 10.1126/sciadv.abd2408
DOI: doi:10.1126/sciadv.abd2408
ISSN: 2375-2548
EISSN: 2375-2548
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Science Advances
Version: Author's manuscript

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