Skip to main content

A Qrr Noncoding RNA Deploys Four Different Regulatory Mechanisms to Optimize Quorum-Sensing Dynamics

Author(s): Feng, Lihui; Rutherford, Steven T.; Papenfort, Kai; Bagert, John D.; van Kessel, Julia C.; et al

To refer to this page use:
Abstract: Quorum sensing is a cell-cell communication process that bacteria use to transition between individual and social lifestyles. In vibrios, homologous small RNAs called the Qrr sRNAs function at the center of quorum-sensing pathways. The Qrr sRNAs regulate multiple mRNA targets including those encoding the quorum-sensing regulatory components luxR, luxO, luxM, and aphA. We show that a representative Qrr, Qrr3, uses four distinct mechanisms to control its particular targets: Qrr3 sRNA represses luxR through catalytic degradation, represses luxM through coupled degradation, represses luxO through sequestration, and activates aphA by revealing the ribosome-binding site while the sRNA itself is degraded. Qrr3 forms different basepairing interactions with each mRNA target, and the particular pairing strategy determines which regulatory mechanism occurs. Combined mathematical modeling and experiments show that the specific Qrr regulatory mechanism employed governs the potency, dynamics and competition of target mRNA regulation, which in turn, defines the overall quorum-sensing response.
Publication Date: 15-Jan-2015
Citation: Feng, Lihui, Rutherford, Steven T, Papenfort, Kai, Bagert, John D, van Kessel, Julia C, Tirrell, David A, Wingreen, Ned S, Bassler, Bonnie L. (2015). A Qrr Noncoding RNA Deploys Four Different Regulatory Mechanisms to Optimize Quorum-Sensing Dynamics. Cell, 160 (1-2), 228 - 240. doi:10.1016/j.cell.2014.11.051
DOI: doi:10.1016/j.cell.2014.11.051
ISSN: 0092-8674
Pages: 228 - 240
Type of Material: Journal Article
Journal/Proceeding Title: Cell
Version: Author's manuscript

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.