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|Abstract:||The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 30 untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo’s RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo’s functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.|
|Citation:||Tamayo, Joel V, Teramoto, Takamasa, Chatterjee, Seema, Hall, Traci M Tanaka, Gavis, Elizabeth R. (2017). The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition. Cell Reports, 19 (1), 150 - 161. doi:10.1016/j.celrep.2017.03.022|
|Pages:||1 - 13|
|Type of Material:||Journal Article|
|Journal/Proceeding Title:||Cell Reports|
|Version:||Final published version. Article is made available in OAR by the publisher's permission or policy.|
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